Circulating miR-181c-5p and miR-497-5p are potential biomarkers for prognosis and diagnosis of osteoporosis.
J Clin Endocrinol Metab. 2019 Dec 24;: Authors: Ma J, Lin X, Chen C, Li S, Zhang S, Chen Z, Li D, Zhao F, Yang C, Qiu W, Xiao Y, Zhang K, Miao Z, Yang T, Qian A
CONTEXT: Osteoporosis is a degenerative bone disease in aging men and women. MiRNAs associated with progressive bone loss in osteoporosis had not been clearly demonstrated. OBJECTIVE: Evaluation of the differentially expressed miRNAs in bone tissue and serum of osteoporotic women with aging. METHODS: MiRNAs GeneChip and real-time PCR were used to screen differently expressed miRNAs in bone tissues of 21 aged osteoporotic women at 60-69 years and 80-89 years. Identified miRNAs were detected in serum of validation cohort consisted of 14 healthy premenopausal women and 86 postmenopausal women with osteopenia or osteoporosis. MiR-181c-5p and miR-497-3p expression were validated in aging and OVX mice models, and osteoblasts. Their role in osteogenesis was validated in vitro. RESULTS: 24 miRNAs were found to be top differentially expressed in bone tissues of osteoporotic women in initial screening. 4 miRNAs were identified both in bone tissue and serum in the validation cohort. Among them, miR-181c-5p and miR-497-5p were decreased in the serum of postmenopausal women with osteopenia or osteoporosis, but increased in subjects treated with bisphosphonate plus calcitriol. MiR-181c-5p and miR-497-5p were significantly downregulated in bone tissue of aging and OVX mice models, and upregulated in osteogenic differentiation of hFOB1.19 and MC3T3-E1 cells. Overexpression of miR-181c-5p and miR-497-5p could promote the differentiation and mineralization of osteoblast. CONCLUSIONS: MiR-181c-5p and miR-497-5p are involved in bone metabolism and associated with progressive bone loss of osteoporosis, suggesting that circulating miR-181c-5p and miR-497-5p might act as potential biomarkers for monitoring effects of anti-osteoporosis therapies or diagnostic approach. PMID: 31872255 [PubMed - as supplied by publisher]